Speaker: Stephen Soonthornvacharin

SBMRI Infectious and Inflammatory Disease Center – Sumit Chanda Lab

Global analysis of host-pathogen interactions that regulate early stage HIV-1 replication

Cell 2008 Chanda

Renate Koenig, Sumit Chanda et al.
Cell. 135(1): 49–60 (2008)

Human Immunodeficiency Viruses (HIV-1 and HIV-2) rely upon host-encoded proteins to facilitate
their replication. Here we combined genome-wide siRNA analyses with interrogation of human
interactome databases to assemble a host-pathogen biochemical network containing 213 confirmed
host cellular factors and 11 HIV-1-encoded proteins. Protein complexes that regulate ubiquitin
conjugation, proteolysis, DNA damage response and RNA splicing were identified as important
modulators of early stage HIV-1 infection. Additionally, over 40 new factors were shown to
specifically influence initiation and/or kinetics of HIV-1 DNA synthesis, including cytoskeletal
regulatory proteins, modulators of post-translational modification, and nucleic acid binding proteins.
Finally, fifteen proteins with diverse functional roles, including nuclear transport, prostaglandin synthesis, ubiquitination, and transcription, were found to influence nuclear import or viral DNA integration. Taken together, the multi-scale approach described here has uncovered multiprotein virus-host interactions that likely act in concert to facilitate early steps of HIV-1 infection.

Presenter: Dr. Fulai Jin (UCSD School of Medicine)

UCSD Cellular and Molecular Medicine – Bing Ren Lab

A high-resolution map of signaling dependent, dynamic chromatic interactome in human cells

Fulai Jin, Bing Ren et al.
Nature. (in press)