Conferences & Workshops

Frontiers in Single Cell Biology – SBP’s 38th Annual Symposium
Tuesday, October 10, 2017
9:00 am – 5:00 pm
Hilton Torrey Pines
Hosted by Drs. Peter Adams and Sumit Chanda
Register for the event here

SDCSB’s Quarterly Systems-to-Synthesis Meeting (Fall 2017)
Wednesday, November 1, 2017
2:00 – 5:00 pm
UC San Diego, Medical Education Telemedicine Bldg, 141-143
Registration begins fall 2017

SDCSB’s Quarterly Systems-to-Synthesis Meeting (Spring 2018)
Thursday, May 17, 2018
2:00 – 5:00 pm
UC San Diego, Medical Education Telemedicine Bldg, 141-143
Registration begins spring 2018

 

Weekly Events

Genetics, Bioinformatics and Systems Biology Colloquium (2017-18)

qBio Seminar Series (2017-18)

Systems Biology Career Development Seminars (Spring 2018)

Monthly Events

San Diego Bioinformatics Users Series (SDBUS)

Systems Biology Club

Whole-cell Proteome Dynamics

Investigators: Jeff Hasty, Terry Hwa, Eric Bennett, Nan Hao, Lev Tsimring

Protein abundance levels are controlled through regulatory processes that govern their synthesis and degradation. Although  translational control is now a widely appreciated mechanism for gene regulation and proteome remodeling, a systems level description of the effects of translational regulation on cellular phenotype remains unknown. Previous approaches have used the rates of translation and degradation under steady state conditions to generate deterministic models of protein biogenesis. Yet how cellular proteome flux responds to environmental changes remains poorly understood. It is clear though that limiting nutrient availability or changing carbon source utilization can alter translation and degradation rates. This project’s goal is to discover the processes that regulate and adapt proteome flux to environmental change and to use this knowledge to  predict the cellular response to such changes.

Understanding how proteome structure influences gene expression dynamics will be crucial for a systems level understanding of cellular adaptations to dynamic environments. Since all mRNA’s share the same pool of translational components, an inherent coupling exists between mRNA translation rates in the cell. This coupling can become especially pronounced during periods of rapid environmental change. For example, when nutrient availability changes, the cell must balance its changing access to nutrients with its available anabolic pathway capacities. Through the process of evolution cells have developed a coordinated system that regulates transcription, ribosome biogenesis, translational capacity, protein synthesis and doubling time depending on nutrient availability. Determining how nutrient dynamics affect the cell’s translational capacity will reveal numerous details of the coordination between these various sub-systems and how they are tuned to adapt a cell to new environmental conditions. To achieve a rigorous description of these interactions we will combine theoretical modeling with experimental validation to quantitatively describe system wide cellular adaptations to changes in nutrient availability.

This project will deliver a broad understanding of how nutrient availability alters cellular proteome flux, with relevance to both the basic and applied life sciences. This knowledge will arise through a maps-to-models approach that incorporates transcription, translation and degradation to describe metabolic pathway dynamics. The modeling and experimental approaches described here follow closely from work performed by the project leaders during the first funding period of the SDCSB. In previously published work funded by the Center, we have demonstrated how overloading of protein degradation machinery can lead to coupling across genetic circuits (Prindle et al., Nature 2014) and how mRNA transcripts can compete for limited translational resources, especially in dynamic environments (Behar et al., Cell 2013). This previous work is directly applicable to our current research efforts.  

 

 


 

Recent SDCSB Publications by these Investigators:

  1. Scott, SR, Din, MO, Bittihn, P, Xiong, L, Tsimring, LS, Hasty, J et al.. A stabilized microbial ecosystem of self-limiting bacteria using synthetic quorum-regulated lysis. Nat Microbiol. 2017;2 :17083. doi: 10.1038/nmicrobiol.2017.83. PubMed PMID:28604679 .
  2. Didovyk, A, Tsimring, LS. Synthetic Gene Circuits Learn to Classify. Cell Syst. 2017;4 (2):151-153. doi: 10.1016/j.cels.2017.02.001. PubMed PMID:28231449 PubMed Central PMC5475398.
  3. Sundaramoorthy, E, Leonard, M, Mak, R, Liao, J, Fulzele, A, Bennett, EJ et al.. ZNF598 and RACK1 Regulate Mammalian Ribosome-Associated Quality Control Function by Mediating Regulatory 40S Ribosomal Ubiquitylation. Mol. Cell. 2017;65 (4):751-760.e4. doi: 10.1016/j.molcel.2016.12.026. PubMed PMID:28132843 PubMed Central PMC5321136.
  4. Bittihn, P, Hasty, J, Tsimring, LS. Suppression of Beneficial Mutations in Dynamic Microbial Populations. Phys. Rev. Lett. 2017;118 (2):028102. doi: 10.1103/PhysRevLett.118.028102. PubMed PMID:28128631 PubMed Central PMC5552243.
  5. Humphries, J, Xiong, L, Liu, J, Prindle, A, Yuan, F, Arjes, HA et al.. Species-Independent Attraction to Biofilms through Electrical Signaling. Cell. 2017;168 (1-2):200-209.e12. doi: 10.1016/j.cell.2016.12.014. PubMed PMID:28086091 PubMed Central PMC5497501.
  6. Dai, X, Zhu, M, Warren, M, Balakrishnan, R, Patsalo, V, Okano, H et al.. Reduction of translating ribosomes enables Escherichia coli to maintain elongation rates during slow growth. Nat Microbiol. 2016;2 :16231. doi: 10.1038/nmicrobiol.2016.231. PubMed PMID:27941827 PubMed Central PMC5346290.
  7. AkhavanAghdam, Z, Sinha, J, Tabbaa, OP, Hao, N. Dynamic control of gene regulatory logic by seemingly redundant transcription factors. Elife. 2016;5 :. doi: 10.7554/eLife.18458. PubMed PMID:27690227 PubMed Central PMC5047750.
  8. Cremer, J, Segota, I, Yang, CY, Arnoldini, M, Sauls, JT, Zhang, Z et al.. Effect of flow and peristaltic mixing on bacterial growth in a gut-like channel. Proc. Natl. Acad. Sci. U.S.A. 2016;113 (41):11414-11419. doi: 10.1073/pnas.1601306113. PubMed PMID:27681630 PubMed Central PMC5068270.
  9. Harper, JW, Bennett, EJ. Proteome complexity and the forces that drive proteome imbalance. Nature. 2016;537 (7620):328-38. doi: 10.1038/nature19947. PubMed PMID:27629639 PubMed Central PMC5204264.
  10. Steiner, PJ, Williams, RJ, Hasty, J, Tsimring, LS. Criticality and Adaptivity in Enzymatic Networks. Biophys. J. 2016;111 (5):1078-87. doi: 10.1016/j.bpj.2016.07.036. PubMed PMID:27602735 PubMed Central PMC5018143.
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