Mike Ferry and Jeff Hasty, Bonner Hall 2121

The Cell Dynamics Core develops technology and protocols for live cell imaging in dynamically changing environments.  Fluorescent reporters allow for dynamic data acquisition, microfluidic devices allow users to determine the dynamically changing environment, and live cell microscopy and automated image analysis allows for quantitative dynamic data over long timecourse.

The Core provides microfluidic chips and master molds based on pre-designed masks or user designs, and facilities for live cell imaging in dynamically changing environment.  In addition, the core assists users in fluorescent reporter designs, the conception of microfluidic designs, the implementation of microfabrication technology, fluorescent-reporter quantification, automated live-cell imaging, and computer-assisted image processing.

The Core will promote the use of microfluidic technology within the biomedical research community by presenting seminars describing the fundamentals of microfabrication as well as examples of the successful implementation microfluidic technology at laboratory group meetings.  These seminars will engage laboratory members in discussions about how microfluidic devices can be used to address their specific research needs.

The SDCSB/BCI microscopy core now has a Nikon TE2000-U fully motorized microscope featuring a 14 bit Andor Clara cooled CCD Hi sensitivity monochrome camera with 1392 x 1040 pixel resolution.  The microscope features a motorized XY stage with linear encoders for superb positional accuracy and an automated autofocus routine controlled by the Nikon Elements AR software package.  The microscope is fully optimized for long duration time lapse microscopy using microfluidic devices and includes two IAI Robocylinder linear actuators for dial-a-wave microfluidic experiments.  The microscope also has an incubator box for precise temperature control during experiments.

This microscope is dedicated to users of the core and all those interested in using it should complete the online form HERE to schedule an orientation on its use.